Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways.

Abstract

The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5'-untranslated region of the CM-ACO1 gene was fused to the beta-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment.

• Authors: T Bouquin, E Lasserre, J Pradier, J C Pech, C Balagué
• Journal: Plant molecular biology (Plant Mol Biol) Vol. 35 Issue 6 Pg. 1029-35 (Dec 1997) ISSN: 0167-4412 [Print] Netherlands
• PMID: 9426625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Amino Acids, Cyclic
• Ethylenes
• 1-aminocyclopropane-1-carboxylic acid
• ethylene
• Amino Acid Oxidoreductases
• 1-aminocyclopropane-1-carboxylic acid oxidase

Topics

• Amino Acid Oxidoreductases (biosynthesis, genetics)
• Amino Acids (biosynthesis, genetics)
• Amino Acids, Cyclic
• Cucurbitaceae (enzymology, genetics)
• Enzyme Induction (drug effects)
• Ethylenes (pharmacology)
• Gene Expression Regulation, Enzymologic (drug effects)
• Gene Expression Regulation, Plant (drug effects)
• Genes, Plant
• Plant Leaves (enzymology, genetics)
• Signal Transduction (drug effects, genetics)