Immunodominant RNA recognition motifs of human nucleolin/C23.

Abstract

Nucleolin/C23 is a nucleolar phosphoprotein implicated in the synthesis, processing and transport of ribosomal RNA and gene transcription. Auto-antibodies to human nucleolin/C23 have been reported in patients with systemic lupus erythematosus and other systemic autoimmune disorders. To identify immunodominant regions in nucleolin/C23, deletion fragments of nucleolin/C23 were fused in frame with the glutathione S-transferase gene. Seven monoclonal anti-nucleolin/C23 antibodies were used to determine the immunoreactivity of the bacterially expressed fusion proteins. Two sets of immunogenic regions at amino acids 314-389 and 387-461 were identified; each contained overlapping discontinuous epitopes and a centrally located RNA recognition motif. An auto-immune serum from a patient with systemic lupus erythematosus patient was found to contain antibodies against human nucleolin/C23 which recognized amino acids 387-461 of nucleolin/C23.

• Authors: B C Valdez, D Henning, R K Busch, M Srivastava, H Busch
• Journal: Molecular immunology (Mol Immunol) Vol. 32 Issue 16 Pg. 1207-13 (Nov 1995) ISSN: 0161-5890 [Print] England
• PMID: 8559145 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Epitopes
• Nuclear Proteins
• Phosphoproteins
• RNA-Binding Proteins
• nucleolin
• RNA

Topics

• Amino Acid Sequence
• Antibodies, Monoclonal (immunology)
• Epitope Mapping
• Epitopes (genetics, immunology)
• Gene Deletion
• HeLa Cells
• Humans
• Molecular Sequence Data
• Nuclear Proteins (genetics, immunology, metabolism)
• Phosphoproteins (genetics, immunology, metabolism)
• RNA (metabolism)
• RNA-Binding Proteins