Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis.

Abstract

The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis. [formula: see text]

• Authors: A Klein, C Carnoy, G Lamblin, P Roussel, J A van Kuik, J F Vliegenthart
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 211 Issue 3 Pg. 491-500 (Feb 01 1993) ISSN: 0014-2956 [Print] England
• PMID: 8436110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycoproteins
• Oligosaccharides
• Sugar Alcohols
• sialooligosaccharides
• Pronase

Topics

• Bronchiectasis (metabolism)
• Carbohydrate Sequence
• Chromatography, Gel
• Chromatography, High Pressure Liquid
• Chromatography, Ion Exchange
• Glycoproteins (chemistry)
• Humans
• Hydrogen-Ion Concentration
• Magnetic Resonance Spectroscopy
• Methylation
• Molecular Sequence Data
• Mucus (chemistry)
• Oligosaccharides (chemistry, isolation & purification)
• Pronase (metabolism)
• Sugar Alcohols (chemistry, isolation & purification)