Vitamin K-dependent carboxylase activity, prothrombin mRNA, and prothrombin production in two cultured rat hepatoma cell lines.

Abstract	The presence of under-gamma-carboxylated forms of plasma prothrombin is a marker for human primary hepatocellular carcinoma. A rat hepatoma cell line (7777) which was previously shown to secrete undercarboxylated prothrombin when grown as a solid tumor has now been grown in monolayer culture. This cell line has a decreased activity of the microsomal vitamin K-dependent carboxylase when compared to a control (H4IIEC3) hepatoma line, does not increase intracellular prothrombin concentrations in response to vitamin K depletion, and secretes undercarboxylated prothrombin even when grown in vitamin K supplemented media. Prothrombin gene expression in the 7777 cell line, as measured by prothrombin mRNA levels, was not altered in the 7777 cell line. This cell line appears to be a model for assessing the cellular alterations responsible for undercarboxylated prothrombin excretion by human hepatocellular tumors.
Authors	D V Shah, P Zhang, J A Engelke, A U Bach, J W Suttie
Journal	Thrombosis research (Thromb Res) Vol. 70 Issue 5 Pg. 365-73 (Jun 01 1993) ISSN: 0049-3848 [Print] United States
PMID	8378893 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Biomarkers Neoplasm Proteins Phosphatidylcholines RNA, Messenger RNA, Neoplasm Vitamin K Warfarin Prothrombin Factor X Ligases Carbon-Carbon Ligases glutamyl carboxylase Ammonium Sulfate
Topics	Alkylation (drug effects) Amino Acid Sequence Ammonium Sulfate (pharmacology) Animals Biomarkers Carbon-Carbon Ligases Factor X (pharmacology) Gene Expression Regulation, Neoplastic Ligases (metabolism) Liver Neoplasms, Experimental (metabolism, pathology) Male Molecular Sequence Data Neoplasm Proteins (genetics, metabolism) Phosphatidylcholines (pharmacology) Protein Processing, Post-Translational (drug effects) Prothrombin (biosynthesis, genetics, metabolism) RNA, Messenger (genetics) RNA, Neoplasm (genetics) Rats Rats, Inbred BUF (blood) Tumor Cells, Cultured (drug effects) Vitamin K (pharmacology) Warfarin (pharmacology)

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