Synovial cells are potent antigen-presenting cells for superantigen, staphylococcal enterotoxin B (SEB).
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| Abstract |
There is ample evidence suggesting that superantigens may act as a triggering factor in the pathogenesis of rheumatoid arthritis (RA). We investigated whether superantigen could activate T cells in the presence of synovial cells. T cells were cultured with SEB in the presence of interferon-gamma (IFN-gamma)-treated synovial cells. T cell proliferation and activation were assessed by 3H-thymidine incorporation and IL-2 production. The expression of HLA class II antigens and adhesion molecules on synovial cells was detected by flow cytometer. In the presence of IFN-gamma-treated synovial cells, T cells proliferated vigorously and produced IL-2 in response to SEB. A low SEB-induced T cell response was noticed in the presence of untreated synovial cells. Allogeneic as well as autologous IFN-gamma-treated synovial cells markedly enhanced SEB-induced T cell proliferation. IFN-gamma-treated synovial cells had increased expression of HLA class II antigens and intercellular adhesion molecule-1 (ICAM-1) adhesion molecules. MoAbs towards these antigens markedly inhibited the SEB-induced T cell response. These results indicate that activated synovial cells are potent antigen-presenting cells for SEB to T cells, and that superantigens may play a critical role in the pathogenesis of RA through activated synovial cells.
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| Authors | T Origuchi, K Eguchi, Y Kawabe, A Mizokami, H Ida, S Nagataki |
| Journal | Clinical and experimental immunology (Clin Exp Immunol) Vol. 99 Issue 3 Pg. 345-51 (Mar 1995) ISSN: 0009-9104 [Print] England |
| PMID | 7882555 (Publication Type: Journal Article) |
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