Alteration of cell surface
carbohydrate antigens during malignant transformation is a well-known phenomenon observed in various
tumors. In prostatic
carcinoma, nearly total deletion of normally occurring ABO and type I-based
Lewis antigens, Le(a) and Leb, has been observed in several studies. We studied expression of the closely related type II
antigens Le(x), Le(y), and
sialyl-Lewis(x) (
SLe(x)) using
monoclonal antibodies. Thirty
formalin-fixed specimens obtained from radical
prostatectomy, containing prostatic
carcinoma as well as benign tissue, were evaluated by immunohistochemistry. In both
cancer and benign tissue, Le(x) expression was minimal or absent. In benign tissue, Le(y) was expressed in ducts and in the basal layer of glandular epithelium. In
tumor tissue, Le(y) expression was greatly increased and extensive staining was observed in 26 of 30 cases. The
SLe(x) expression in benign tissue was observed only in larger ducts, never in glandular secretory epithelial cells. In
carcinoma, rare cells positive for
SLe(x) were present in 8 of 30 cases, and stronger expression with focal to patchy distribution was observed in 14 of 30 cases. The results suggest an alteration in glycosyl
transferase activity in prostatic
carcinoma, with preserved or increased activity of
enzymes responsible for the synthesis of the type II core sequence. This sequence is further glycosylated and expressed as the difucosylated compound Le(y) or the monofucosyl, monosialyl compound
SLe(x). For prostate, Le(y) and
SLe(x) are the only
blood group-related
antigens known to be minimal or absent in benign secretory epithelial cells that are more highly expressed in malignant tissue.(ABSTRACT TRUNCATED AT 250 WORDS)