Isolation, identification, and characterization of clones encoding antigens responsible for peanut hypersensitivity.
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| Abstract |
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using Ara h I-specific oligonucleotides and polymerase chain reaction technology. The Ara h I clone identified a 2.3-kb mRNA species on a Northern blot containing peanut poly A+RNA. DNA sequence analysis of the cloned inserts revealed that the Ara h I allergen has significant homology with the vicilin seed storage protein family found in most higher plants. The isolation of the Ara h I clones allowed the synthesis of this protein in Escherichia coli cells and subsequent recognition of this recombinant protein in immunoblot analysis using serum IgE from patients with peanut hypersensitivity. With the production of the recombinant peanut protein it will now be possible to address the pathophysiologic and immunologic mechanisms regarding peanut hypersensitivity reactions specifically and food hypersensitivity in general.
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| Authors | A W Burks, G Cockrell, J S Stanley, R M Helm, G A Bannon |
| Journal | International archives of allergy and immunology (Int Arch Allergy Immunol) 1995 May-Jun Vol. 107 Issue 1-3 Pg. 248-50 ISSN: 1018-2438 [Print] Switzerland |
| PMID | 7613142 (Publication Type: Comparative Study, Journal Article, Review) |
| Chemical References |
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