In vitro and in vivo antileukemic activity of B43-pokeweed antiviral protein against radiation-resistant human B-cell precursor leukemia cells.

Abstract	B-cell precursor (BCP) leukemia is the most common form of childhood cancer and represents one of the most radiation-resistant forms of human malignancy. In this study, we examined the antileukemic efficacy of the B43 (anti-CD19)-pokeweed antiviral protein (B43-PAP) immunotoxin against radiation-resistant BCP leukemia cells. B43-PAP caused apoptosis of radiation-resistant primary BCP leukemia cells, killed greater than 99% of radiation-resistant primary leukemic progenitor cells from BCP leukemia patients, and conferred extended survival to severe combined immunodeficiency (SCID) mice xenografted with radiation-resistant human BCP leukemia. Furthermore, the combination of B43-PAP and total body irradiation (TBI) was more effective than TBI alone in two SCID mouse bone marrow transplantation models of radiation-resistant human BCP leukemia. Thus, B43-PAP may prove useful in the treatment of radiation-resistant BCP leukemia.
Authors	K G Waddick, D E Myers, R Gunther, L M Chelstrom, M Chandan-Langlie, J D Irvin, N Tumer, F M Uckun
Journal	Blood (Blood) Vol. 86 Issue 11 Pg. 4228-33 (Dec 01 1995) ISSN: 0006-4971 [Print] United States
PMID	7492781 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antineoplastic Agents, Phytogenic Antiviral Agents Immunotoxins Plant Proteins Ribosome Inactivating Proteins, Type 1 N-Glycosyl Hydrolases pokeweed antiviral protein
Topics	Animals Antineoplastic Agents, Phytogenic (pharmacology) Antiviral Agents (pharmacology) Apoptosis (drug effects, radiation effects) Bone Marrow Transplantation Burkitt Lymphoma (drug therapy, radiotherapy, surgery) Child Combined Modality Therapy Humans Immunotoxins (pharmacology) In Vitro Techniques Mice Mice, SCID N-Glycosyl Hydrolases Neoplasm Transplantation Neoplastic Stem Cells (drug effects, radiation effects) Plant Proteins (pharmacology) Radiation Tolerance Ribosome Inactivating Proteins, Type 1 Transplantation, Heterologous Transplantation, Isogeneic Whole-Body Irradiation

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