The number of lymphocytes forming spontaneous rosettes with sheep erythrocytes, a property of thymus-dependent (T) cells, and the number of lymphocytes bearing
surface immunoglobulins, a characteristic feature of bone marrow-dependent (B) cells, were determined in the peripheral blood of normals and of patients with
chronic lymphocytic leukemia (CLL) and
Hodgkin's disease. As compared with normal individuals CLL patients had an increased percentage of lymphocytes with
membrane-bound immunoglobulins, whereas the proportion of rosette-forming lymphocytes was reduced. In
Hodgkin's disease either normal, diminished, or increased B cell values were obtained; the percentage of T cells was decreased or within the lower range of normals. Lymphocyte transformation by various mitogenic agents in vitro may be regarded as a model of lymphocyte reactivity during immunologic processes in vivo. In order to study the functional capacity of lymphocytes in CLL and
Hodgkin's disease in comparison with normal cells, purified peripheral blood lymphocytes from normals and patients with these diseases were incubated in vitro with
phytohemagglutinin (PHA) and
pokeweed mitogen (PWM) over 7 to 11 days.
DNA synthesis was determined by incorporation of 3-H-thymidine. The cyto-architectural features of the cells before and during incubation with these
phytomitogens were studied by electron microscopy. Planimetric measurements were performed on micrographs of comparable cell sections (through nucleus and Golgi zone) for the determiniation of cell, nuclear, cytoplasmic, and mitochondrial area. Furthermore, the number of mitochondria and of membrane-bounded
acid phosphatase-positive lysosome-like organelles was determined in comparable sections of unstimulated and
mitogen transformed lymphocytes.