Abstract |
A radioimmunoassay using monoclonal antibodies (MAb) against 0(6)-medGuo and 0(6)-etdGuo enabled the presence of these DNA adducts to be detected in human tissues. With this methodology and with 1 mg of hydrolysed DNA it is possible to detect approximately 25 fmol 0(6)-medGuo or 12.5 fmol 0(6)-etdGuo per mg DNA. The analysis comprised a total of 37 human tissue specimens derived from patients who underwent surgery for cancer of the oesophagus in Linxian County (People's Republic of China); for comparison 12 tissue samples obtained from hospitals in Europe were similarly analysed. Seventeen samples from Linxian County showed a level of 0(6)-medGuo ranging from 15 to 50 fmol/mg DNA, 10 showed higher levels up to 160 fmol/mg DNA, and the remaining 10 samples were below the limit of detection. All the tissue samples from Europe showed levels below 45 fmol 0(6)-medGuo/mg DNA, 7 being below the limit of detection. No 0(6)-etdGuo was detected in any of the samples. 0(6)-alkylguanine DNA alkyltransferase activity observed in tissue extracts from Linxian County ranged from 190 to 326 fmol (mean values) of 0(6)-medGuo removed per mg protein and lower values were obtained for the removal of 0(6)-etdGuo. The findings obtained using this approach appear promising in assessing the role of alkylating agents, e.g. nitrosamines, in the aetiology of human cancer.
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Authors | D Umbenhauer, C P Wild, R Montesano, R Saffhill, J M Boyle, N Huh, U Kirstein, J Thomale, M F Rajewsky, S H Lu |
Journal | International journal of cancer
(Int J Cancer)
Vol. 36
Issue 6
Pg. 661-5
(Dec 15 1985)
ISSN: 0020-7136 [Print] United States |
PMID | 4066072
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nitrosamines
- DNA
- O(6)-methyl-2'-deoxyguanosine
- Methyltransferases
- O(6)-Methylguanine-DNA Methyltransferase
- Deoxyguanosine
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Topics |
- China
- DNA
(analysis)
- Deoxyguanosine
(analogs & derivatives, analysis)
- Esophageal Neoplasms
(chemically induced, metabolism)
- Esophagus
(metabolism)
- France
- Gastric Mucosa
(metabolism)
- Germany
- Humans
- Methyltransferases
(metabolism)
- Mucous Membrane
(metabolism)
- Nitrosamines
(metabolism)
- O(6)-Methylguanine-DNA Methyltransferase
- Radioimmunoassay
- Risk
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