Potentiation of collagen synthesis in explants of the rabbit eye by 5 beta-dihydrocortisol.

Abstract

The biologic effect of 5 beta-dihydrocortisol on collagen synthesis was evaluated. The metabolite was found to potentiate subthreshold levels of dexamethasone in increasing 3H-proline incorporation in cells of the outflow region of the rabbit. Digestion of the tissue with highly purified collagenase indicated that the 3H-proline was incorporated into collagen type protein. This study demonstrates another biologic activity of 5 beta-dihydrocortisol, a metabolite found to accumulate in cells cultured from trabeculectomy specimens from patients with primary open angle glaucoma.

• Authors: A L Southren, M R Hernandez, D l'Hommedieu, G G Gordon, B I Weinstein
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 27 Issue 12 Pg. 1757-60 (Dec 1986) ISSN: 0146-0404 [Print] United States
• PMID: 3793407 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Tritium
• 5-dihydrocortisol
• Collagen
• Proline
• Hydrocortisone

Topics

• Animals
• Autoradiography
• Collagen (biosynthesis)
• Eye (metabolism)
• Hydrocortisone (analogs & derivatives, pharmacology)
• In Vitro Techniques
• Proline (metabolism)
• Rabbits
• Tritium