Utility of Coproporphyrin-I Determination in First-in-Human Study for Early Evaluation of OATP1B Inhibitory Potential Based on Investigation of Ensitrelvir, an Oral SARS-CoV-2 3C-Like Protease Inhibitor.

Abstract

Coproporphyrin-I (CP-I) has been investigated as an endogenous biomarker of organic anion transporting polypeptide (OATP) 1B. Here, we determined the CP-I concentrations in a cocktail drug-drug interaction (DDI) study of ensitrelvir to evaluate the OATP1B inhibitory potential because ensitrelvir had increased plasma concentrations of rosuvastatin in this study, raising concerns about breast cancer resistance protein and OATP1B inhibition. Furthermore, CP-I concentrations were compared between active and placebo groups in a first-in-human (FIH) study of ensitrelvir to verify whether the OATP1B inhibitory potential could be estimated at an early drug development stage. In the cocktail DDI study, CP-I did not differ between with/without administration of ensitrelvir, indicating that ensitrelvir has no OATP1B inhibitory effect. Although there were some individual variabilities in CP-I concentrations among the treatment groups in the FIH study, the normalization of CP-I concentrations with pre-dose values minimized these variabilities, suggesting that this normalized method would be helpful for comparing the CP-I from different participants. Finally, we concluded that CP-I concentrations were not affected by ensitrelvir in the FIH study. These results suggested that the CP-I determination in an FIH study and its normalized method can be useful for an early evaluation of the OATP1B-mediated DDI potential in humans.

• Authors: Ryosuke Watari, Hiromi Sawada, Hiroshi Hashimoto, Yasuyuki Kasai, Ryoko Oka, Ryosuke Shimizu, Takanobu Matsuzaki
• Journal: Journal of pharmaceutical sciences (J Pharm Sci) Vol. 113 Issue 3 Pg. 798-805 (03 2024) ISSN: 1520-6017 [Electronic] United States
• PMID: 37742997 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.

Chemical References

• ensitrelvir
• ATP Binding Cassette Transporter, Subfamily G, Member 2
• Protease Inhibitors
• Coproporphyrins
• Liver-Specific Organic Anion Transporter 1
• Neoplasm Proteins
• Enzyme Inhibitors
• Anti-Infective Agents
• Antiviral Agents
• Indazoles
• Triazines
• Triazoles

Topics

• Humans
• ATP Binding Cassette Transporter, Subfamily G, Member 2
• SARS-CoV-2
• Protease Inhibitors
• Coproporphyrins (metabolism, pharmacology)
• Liver-Specific Organic Anion Transporter 1 (metabolism)
• COVID-19
• Neoplasm Proteins (metabolism)
• Enzyme Inhibitors
• Anti-Infective Agents
• Antiviral Agents (pharmacology)
• Drug Interactions
• Indazoles
• Triazines
• Triazoles