Cryotherapy is a common non-pharmacological method to relieve
pain and
inflammation. Clinical studies have shown that
cryotherapy can reduce
postoperative pain after
root canal therapy, but the mechanism remains unclear. In this study, we aimed to investigate the underlying molecular mechanisms by which
cryotherapy reduces
inflammation in
lipopolysaccharide (LPS)-stimulated periodontal ligament cells through transcriptome sequencing analysis. We found that
cryotherapy significantly reduced the expression of multiple proinflammatory
cytokines and
chemokines, and NFKB1 was the key regulator down-regulated by
cryotherapy. Importantly, we discovered that
lncRNA SNHG1 expression level significantly decreased after cold treatment. SNHG1 expression was positively related to NFKB1 while negatively correlated with miR-9-5p, which formed a novel
ceRNA regulatory pathway. Knockdown of SNHG1 significantly reduced the expression of NFKB1, IL1B, and
IL6, while overexpression of SNHG1 significantly increased the expression of these genes. In conclusion, our study demonstrated that
cryotherapy can effectively reduce
inflammation in LPS-induced periodontal ligament cells by suppressing the
lncRNA SNHG1/miR-9-5p/NFKB1 axis.