Abstract | BACKGROUND: METHODS: C57BL/6J female mice were exposed to total body irradiation (TBI) and partial body irradiation (PBI). Changes in haptoglobin (Hp) level in plasma were shown at different doses and time points after the exposure and treatment with amifostine or bone marrow transplantation. Student's t-test/two tailed test were used in two groups. To decide the Hp levels as a predictor of the radiation dose in TBI and PBI, multiple linear regression analysis were performed. The ability of biomarkers to identify two groups of different samples was determined by the receiver operating characteristic (ROC) curve. The results were expressed as mean ± standard deviation (SD). Significance was set at P value < 0.05, and P value < 0.01 was set as highly significant. Survival distribution was determined by log-rank test. RESULTS: In this study, we found that Hp was elevated dose-dependently in plasma in the early post-irradiation period and decreased on the second day, which can be used as a molecular indicator for early dose assessment. Moreover, we detected the second increase of Hp on the 3rd and 5th days after the lethal irradiation at 10 Gy, which was eliminated by amifostine, a radiation protection drug, while protected mice from death. Most importantly, bone marrow transplantation (BMT) on the 3rd and 5th day after 10 Gy radiation improved the 30-days survival rate, and effectively accelerated the regression of secondary increased Hp level. CONCLUSIONS:
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Authors | Shixiang Zhou, Yaqiong Li, Lexin He, Min Chen, Weihong Li, Ting Xiao, Jian Guan, Zhenhua Qi, Qi Wang, Siyuan Li, Pingkun Zhou, Zhidong Wang |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 13
Issue 1
Pg. 461
(09 06 2022)
ISSN: 1757-6512 [Electronic] England |
PMID | 36068556
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
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Topics |
- Amifostine
- Animals
- Bone Marrow Transplantation
- Female
- Haptoglobins
- Humans
- Mice
- Mice, Inbred C57BL
- Radiation Injuries
(metabolism)
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