Broad-spectrum cannabis oil ameliorates reserpine-induced fibromyalgia model in mice.

Abstract

Fibromyalgia (FM) is an idiopathic disorder characterized by generalized pain and associated symptoms such as depression and anxiety. Cannabis sativa shows different pharmacological activities, such as analgesic, anti-inflammatory, neuroprotective, and immunomodulatory. Associated with this, the use of an oil with low concentrations of THC can reduce the psychomimetic adverse effects of the plant. Therefore, the present study aimed to evaluate the analgesic effect of broad-spectrum cannabis oil with low THC concentration in an experimental model of FM. Mechanical hyperalgesia, thermal allodynia, depressive- and anxious-related behavior, and locomotor activity were evaluated after reserpine (0.25 mg/kg; injected subcutaneously (s.c.) once daily for three consecutive days) administration. Our results showed that oral administration of broad-spectrum cannabis oil (0.1, 1, and 3 mg/kg, p.o.) in a single dose on the 4th day inhibited mechanical hyperalgesia and thermal allodynia induced by reserpine. Relevantly, treatment during four days with broad-spectrum cannabis oil (0.1 mg/kg, p.o.) reduced mechanical hyperalgesia 1 h after reserpine administration. Intraplantar treatment with cannabis oil significantly reversed mechanical and heat thermal nociception induced by reserpine injection. Interestingly, spinal and supraspinal administration of broad-spectrum cannabis oil completely inhibited mechanical hyperalgesia and thermal sensitivity induced by reserpine. The repeated cannabis oil administration, given daily for 14 days, markedly mitigated the mechanical and thermal sensitivity during the FM model, and its reduced depressive-like behavior induced by reserpine. In summary, broad-spectrum cannabis oil is an effective alternative to reverse the reserpine-induced fibromyalgia model.

• Authors: Eduarda Gomes Ferrarini, Rodrigo Sebben Paes, Gabriela Mantovani Baldasso, Pollyana Mendonça de Assis, Murilo Chaves Gouvêa, Paola De Cicco, Nádia Rezende Barbosa Raposo, Raffaele Capasso, Eduardo Luiz Gasnhar Moreira, Rafael Cypriano Dutra
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 154 Pg. 113552 (Oct 2022) ISSN: 1950-6007 [Electronic] France
• PMID: 35988425 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Analgesics
• Dronabinol
• Reserpine

Topics

• Analgesics (adverse effects)
• Animals
• Cannabis
• Disease Models, Animal
• Dronabinol (adverse effects)
• Fibromyalgia (chemically induced, drug therapy)
• Hyperalgesia (chemically induced, complications, drug therapy)
• Mice
• Reserpine (adverse effects)