Ameliorative Effect of Citropten Isolated from Citrusaurantifolia Peel Extract as a Modulator of T Cell and Intestinal Epithelial Cell Activity in DSS-Induced Colitis.

Abstract

Citropten is a coumarin that is mainly found in fruits of Rutaceae trees, but its anti-inflammatory activities in colitis is still unknown. In this study, we investigated its attenuating effect of citropten isolated from Citrus aurantifolia extract on DSS-induced colitis through the modulation of the activity of T cells and intestinal epithelial cells. We found that pre-treatment with citropten downregulates the activity of T cells and intestinal epithelial cells without a negative effect on the viability of Jurkat and HT-29 cells. The results from the Western blot analysis revealed that pre-treatment with citropten reduces the NFκB and MAPK signaling pathway in activated T cells and intestinal epithelial cells. We elucidated that the oral administration of citropten alleviates the colonic inflammation and activity of effector T cells in DSS-induced colitis by measuring changes in body weight, histological scoring from H&E-stained sections, mRNA levels of pro-inflammatory cytokines and the phosphorylation level of the MAPK signaling pathway.

• Authors: Hyun-Su Lee, Eun-Nam Kim, Gil-Saeng Jeong
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 27 Issue 14 (Jul 20 2022) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 35889507 (Publication Type: Journal Article)

Chemical References

• Coumarins
• Cytokines
• Dextran Sulfate
• 5,7-dimethoxycoumarin

Topics

• Animals
• Colitis (chemically induced, drug therapy, metabolism)
• Colon (metabolism)
• Coumarins (pharmacology)
• Cytokines (metabolism)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Epithelial Cells (metabolism)
• Mice
• Mice, Inbred C57BL
• T-Lymphocytes (metabolism)