Abstract |
Sulforaphane, a natural phytochemical compound found in various cruciferous vegetables, has been discovered to present anti- cancer properties. Matrix metalloproteinase-9 (MMP-9) plays a crucial role in gastric cancer metastasis. However, the role of sulforaphane in MMP-9 expression in gastric cancer is not yet defined. Nicotine, a psychoactive alkaloid found in tobacco, is associated with the development of gastric cancer. Here, we found that sulforaphane suppresses the nicotine-mediated induction of MMP-9 in human gastric cancer cells. We discovered that reactive oxygen species (ROS) and MAPKs ( p38 MAPK, Erk1/2) are involved in nicotine-induced MMP-9 expression. AP-1 and NF-κB are the critical transcription factors in MMP-9 expression. ROS/ MAPK (p38 MAPK, Erk1/2) and ROS functioned as upstream signaling of AP-1 and NF-κB, respectively. Sulforaphane suppresses the nicotine-induced MMP-9 by inhibiting ROS-mediated MAPK (p38 MAPK, Erk1/2)/AP-1 and ROS-mediated NF-κB signaling axes, which in turn inhibit cell invasion in human gastric cancer AGS cells. Therefore, the current study provides valuable evidence for developing sulforaphane as a new anti-invasion strategy for human gastric cancer therapy.
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Authors | Shinan Li, Pham Ngoc Khoi, Hong Yin, Dhiraj Kumar Sah, Nam-Ho Kim, Sen Lian, Young-Do Jung |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 23
Issue 9
(May 05 2022)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 35563563
(Publication Type: Journal Article)
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Chemical References |
- Isothiocyanates
- NF-kappa B
- Reactive Oxygen Species
- Sulfoxides
- Transcription Factor AP-1
- Nicotine
- p38 Mitogen-Activated Protein Kinases
- Matrix Metalloproteinase 9
- sulforaphane
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Topics |
- Humans
- Isothiocyanates
- MAP Kinase Signaling System
- Matrix Metalloproteinase 9
(metabolism)
- NF-kappa B
(metabolism)
- Nicotine
(pharmacology)
- Reactive Oxygen Species
(metabolism)
- Stomach Neoplasms
(drug therapy)
- Sulfoxides
- Transcription Factor AP-1
(metabolism)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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