Abstract | INTRODUCTION: METHODS: The obesity mice model was established through feeding mice (C57BL/6J, male, eight-week-old) with high-fat diet and palmitate (PA)-induced cardiomyocyte injury. RNA and protein levels were detected with Quantitative real-time PCR and Western blotting. The levels of TG, TCH, LDL, CK-MB, cTnl, and BNP in the serum or cell medium supernatant were measured using ELISA kits. The ROS level was detected with the DCFH-DA method. Binding sites between different targets were identified using detection of dual luciferase reporter assay. Cell apoptosis was analyzed by flow cytometry. RNA-binding protein immunoprecipitation and chromatin immunoprecipitation were used to detect the binding of DNMT3B with HOTAIR or miR-196b promoter. RESULTS: The expression of HOTAIR was downregulated, and miR-196b was upregulated in the obese myocardial injury. Ghrelin attenuated PA-induced cardiomyocyte injury by increasing HOTAIR. HOTAIR regulated the expression of miR-196b by recruiting DNMT3B to induce methylation of the miR-196b gene promoter. The binding site between miR-196b and IGF-1 was identified. DISCUSSION/CONCLUSION: We demonstrated that ghrelin attenuated PA-induced cardiomyocyte injury by regulating the HOTAIR/miR-196b/IGF-1 signaling pathway. Our findings might provide novel thought for the prevention and treatment of obesity-induced myocardial injury by targeting HOTAIR/miR-196b.
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Authors | Yuan-Yuan Lang, Xin-Yue Xu, Yan-Ling Liu, Chun-Feng Ye, Na Hu, Qing Yao, Wen-Shu Cheng, Zu-Gen Cheng, Yang Liu |
Journal | Obesity facts
(Obes Facts)
Vol. 15
Issue 4
Pg. 540-549
( 2022)
ISSN: 1662-4033 [Electronic] Switzerland |
PMID | 35294947
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 The Author(s). Published by S. Karger AG, Basel. |
Chemical References |
- Ghrelin
- HOTAIR long non-coding RNA, mouse
- MIRN196 microRNA, mouse
- MicroRNAs
- RNA, Long Noncoding
- Insulin-Like Growth Factor I
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Topics |
- Animals
- Epigenesis, Genetic
- Ghrelin
- Insulin-Like Growth Factor I
(genetics, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- MicroRNAs
(genetics)
- Obesity
(complications, genetics)
- RNA, Long Noncoding
(genetics)
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