The anti-cancer role of microRNA-143 in papillary thyroid carcinoma by targeting high mobility group AT-hook 2.

Abstract

Papillary thyroid carcinoma (PTC), a common thyroid cancer (TC) subtype, rapidly increases in occurrence. MicroRNAs (miRNAs), which are non-coding small RNAs, have been demonstrated to play a role in cancer pathogenic mechanisms. Although miR-143 is involved in suppressing certain malignant tumor progression, its biological role is unknown in PTC. The present study found that miR-143 levels were strongly lower in PTC patient samples and cell lines, implying that miR-143 may play a biological role in PTC. Down-regulation of miR-143 resulted in the increased expression of HMGA2. Furthermore, HMGA2 was found to be a direct target of miR-143. A dual-luciferase assay confirmed a direct binding site for miR-143 was confirmed on HMGA2 using a dual-luciferase assay. Next, over-expression of miR-143 suppressed PTC cell growth as analyzed by MTT, clone formation, and Ki-67 immunofluorescence staining assays. miR-143 mimics transfection downregulated the expression of PCNA, CDK4, CDK1, and Cyclin E1. In addition, wound healing and trans-well assays revealed that miR-143 up-regulation inhibited PTC cells invasion and migration. Co-transfection of HMGA2 expression vector restored HMGA2 expression and rescued PTC cells proliferation capability in miR-143 mimics transfected PTC cells, indicating that miR-143 inhibited PTC cells proliferation via HMGA2. These observations were also obtained in xenografts experiments in nude mice. Altogether, our study shed light on miR-143's anti-cancer biological functions in PTC progression through targeting HMGA2, suggesting that restoration of miR-143 could be a potential therapeutic approach for PTC treatment.

• Authors: Chao Ding, Tiefeng Shi, Gang Wu, Jianting Man, Hongyu Han, Yunfu Cui
• Journal: Bioengineered (Bioengineered) Vol. 13 Issue 3 Pg. 6629-6640 (03 2022) ISSN: 2165-5987 [Electronic] United States
• PMID: 35213273 (Publication Type: Journal Article)

Chemical References

• HMGA2 Protein
• HMGA2 protein, human
• MIRN143 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Animals
• Cell Line, Tumor
• Disease Progression
• HMGA2 Protein (genetics, metabolism)
• Humans
• Mice
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Thyroid Cancer, Papillary (genetics, metabolism, pathology)
• Thyroid Gland (pathology)
• Thyroid Neoplasms (genetics, metabolism, pathology)