Abstract |
The harsh urine microenvironment (UME), as an inherent hurdle, endangers and renders urethral repair unreachable. Innovatively, the unfavorable UME is utilized as the design source to construct a UME-responsive 3D-printed hydrogel patch for realizing scarless memory repair, wherein laser-excited reactive oxygen species (ROS) production and mechanical strength elevation using chemically crosslinked silicon quantum dots are accessible. Intriguingly, the proposed composite scaffolds can respond to Ca2+ in urine, cause structure reconfiguration, and repress swelling to further enhance scaffold stiffness. Systematic experiments validate that ROS birth and unexpected stiffness elevation in such UME-responsive scaffolds can realize scarless memory repair of the urethra in vivo. Comprehensive mechanism explorations uncover that the activations of cell proliferation and collagen-related genes (e.g., MMP-1 and COL3A1) and the dampening of fibrosis-related (e.g., TGF-β/Smad) and mechanosensitive genes (e.g., YAP/TAZ) are responsible for the scarless memory repair of such UME-responsive scaffolds via enhancing collagen deposition, recalling mechanical memory, decreasing fibrosis and inflammation, and accelerating angiogenesis. The design rationales (e.g., UME-initiated structure reconfiguration and antiswelling) can serve as an instructive and general approach for urethra repair.
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Authors | Ming Yang, Yang Zhang, Chao Fang, Li Song, Ying Wang, Lu Lu, Ranxing Yang, Zhaoting Bu, Xiayi Liang, Kun Zhang, Qiang Fu |
Journal | Advanced materials (Deerfield Beach, Fla.)
(Adv Mater)
Vol. 34
Issue 14
Pg. e2109522
(Apr 2022)
ISSN: 1521-4095 [Electronic] Germany |
PMID | 35120266
(Publication Type: Journal Article)
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Copyright | © 2022 Wiley-VCH GmbH. |
Chemical References |
- Hydrogels
- Reactive Oxygen Species
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Topics |
- Fibrosis
- Humans
- Hydrogels
(chemistry)
- Male
- Reactive Oxygen Species
- Tissue Scaffolds
(chemistry)
- Urethra
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