Nanofiber/hydrogel core-shell scaffolds with three-dimensional multilayer patterned structure for accelerating diabetic wound healing.

Abstract

Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/hydrogel core-shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15-80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core-shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (D, L-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/hydrogel core-shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing.

• Authors: Jiankai Li, Tianshuai Zhang, Mingmang Pan, Feng Xue, Fang Lv, Qinfei Ke, He Xu
• Journal: Journal of nanobiotechnology (J Nanobiotechnology) Vol. 20 Issue 1 Pg. 28 (Jan 08 2022) ISSN: 1477-3155 [Electronic] England
• PMID: 34998407 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• Hydrogels
• Methacrylates
• gelatin methacryloyl
• Gelatin

Topics

• Cell Adhesion (drug effects)
• Cells, Cultured
• Diabetes Complications (pathology)
• Gelatin
• Humans
• Hydrogels
• Methacrylates
• Nanofibers
• Neovascularization, Physiologic (drug effects)
• Tissue Scaffolds
• Wound Healing (drug effects)