Abstract | OBJECTIVES: METHODS: A total number of 189 Padua University-Hospital healthcare workers (HCW) who had received a second vaccine dose were asked to collect serum samples for determining Ab at 12 (t12) and 28 (t28) days, and 6 months (t6m) after their first Comirnaty/ BNT162b2 inoculation. Ab titers were measured with plaque reduction neutralization test (PRNT), and three chemiluminescent immunoassays, targeting the receptor binding domain (RBD), the trimeric Spike protein (trimeric-S), and surrogate viral neutralization tests (sVNT). RESULTS: The median percentages (interquartile range) for decrease in antibodies values 6 months after the first dose were 86.8% (67.1-92.8%) for S-RBD IgG, 82% (58.6-89.3%) for trimeric-S, 70.4% (34.5-86.4%) for VNT-Nab, 75% (50-87.5%) for PRNT50 and 75% (50-93.7%) for PRNT90. At 6 months, neither PRNT titers nor VNT-Nab and S-RBD IgG bAb levels correlated with age (p=0.078) or gender (p=0.938), while they were correlated with previous infection (p<0.001). CONCLUSIONS: After 6 months, a method-independent reduction of around 90% in anti-SARS-CoV-2 antibodies was detected, while no significant differences were found between values of males and females aged between 24 and 65 years without compromised health status. Further efforts to improve analytical harmonization and standardization are needed.
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Authors | Andrea Padoan, Chiara Cosma, Francesco Bonfante, Foscarina Della Rocca, Francesco Barbaro, Claudia Santarossa, Luigi Dall'Olmo, Matteo Pagliari, Alessio Bortolami, Annamaria Cattelan, Vito Cianci, Daniela Basso, Mario Plebani |
Journal | Clinical chemistry and laboratory medicine
(Clin Chem Lab Med)
Vol. 60
Issue 3
Pg. 456-463
(02 23 2022)
ISSN: 1437-4331 [Electronic] Germany |
PMID | 34911170
(Publication Type: Journal Article)
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Copyright | © 2021 Andrea Padoan et al., published by De Gruyter, Berlin/Boston. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19 Vaccines
- Immunoglobulin G
- BNT162 Vaccine
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Topics |
- Adult
- Aged
- Antibodies, Neutralizing
(blood)
- Antibodies, Viral
(blood)
- BNT162 Vaccine
- COVID-19
(prevention & control)
- COVID-19 Vaccines
(immunology)
- Female
- Humans
- Immunoassay
- Immunoglobulin G
(blood)
- Kinetics
- Male
- Middle Aged
- SARS-CoV-2
- Vaccination
- Young Adult
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