Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.

Abstract

The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment. In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated. The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice. It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression. Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines. Furthermore, sCPA repaired intestinal microbiota composition especially Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria that altered by DSS in colitis mice. Meanwhile, SCFAs produced by gut microbiota were restored by sCPA close to the level in the normal group. In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.

• Authors: Hongyan Li, Hongxia Che, Jingwen Xie, Xiufang Dong, Lin Song, Wancui Xie, Jinyuan Sun
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 195 Pg. 67-74 (Jan 15 2022) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 34896151 (Publication Type: Journal Article)
• Copyright: Copyright © 2021. Published by Elsevier B.V.

Chemical References

• Glucans
• NF-kappa B
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nlrp3 protein, mouse
• Occludin
• Ocln protein, mouse
• Selenium Compounds
• Tjp1 protein, mouse
• Zonula Occludens-1 Protein
• Dextran Sulfate

Topics

• Animals
• Bacteria (classification, drug effects, isolation & purification)
• Colitis (chemically induced, drug therapy, metabolism, microbiology)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Gene Expression Regulation (drug effects)
• Glucans (chemistry)
• Mice
• Mice, Inbred C57BL
• Microbiota (drug effects)
• NF-kappa B (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein (metabolism)
• Occludin (metabolism)
• Oxidative Stress (drug effects)
• Phylogeny
• Selenium Compounds (administration & dosage, chemistry, pharmacology)
• Weight Loss (drug effects)
• Zonula Occludens-1 Protein (metabolism)