N-acetylglucosamine transporter, Ngt1, undergoes sugar-responsive endosomal trafficking in Candida albicans.

Abstract

N-acetylglucosamine (GlcNAc), an important amino sugar at the infection sites of the fungal pathogen Candida albicans, triggers multiple cellular processes. GlcNAc import at the cell surface is mediated by GlcNAc transporter, Ngt1 which seems to play a critical role during GlcNAc signaling. We have investigated the Ngt1 dynamics that provide a platform for further studies aimed at understanding the mechanistic insights of regulating process(es) in C. albicans. The expression of this transporter is prolific and highly sensitive to even very low levels (˂2 µM) of GlcNAc. Under these conditions, Ngt1 undergoes phosphorylation-associated ubiquitylation as a code for internalization. This ubiquitylation process involves the triggering proteins like protein kinase Snf1, arrestin-related trafficking adaptors (ART) protein Rod1, and yeast ubiquitin ligase Rsp5. Interestingly, analysis of ∆snf1 and ∆rsp5 mutants revealed that while Rsp5 is promoting the endosomal trafficking of Ngt1-GFPɤ, Snf1 hinders the process. Furthermore, colocalization experiments of Ngt1 with Vps17 (an endosomal marker), Sec7 (a trans-Golgi marker), and a vacuolar marker revealed the fate of Ngt1 during sugar-responsive endosomal trafficking. ∆ras1 and ∆ubi4 mutants showed decreased ubiquitylation and delayed endocytosis of Ngt1. According to our knowledge, this is the first report which illustrates the mechanistic insights that are responsible for endosomal trafficking of a GlcNAc transporter in an eukaryotic organism.

• Authors: Kongara Hanumantha Rao, Kasturi Roy, Soumita Paul, Swagata Ghosh
• Journal: Molecular microbiology (Mol Microbiol) Vol. 117 Issue 2 Pg. 429-449 (02 2022) ISSN: 1365-2958 [Electronic] England
• PMID: 34877729 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 John Wiley & Sons Ltd.

Chemical References

• Endosomal Sorting Complexes Required for Transport
• Fungal Proteins
• Sugars
• Phosphoenolpyruvate Sugar Phosphotransferase System
• acetylglucosamine transporter
• Acetylglucosamine

Topics

• Acetylglucosamine (metabolism)
• Candida albicans (genetics, metabolism)
• Endocytosis
• Endosomal Sorting Complexes Required for Transport (genetics, metabolism)
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Phosphoenolpyruvate Sugar Phosphotransferase System (metabolism)
• Sugars (metabolism)