Aptamers targeting a tumor-associated extracellular matrix component: The human mature collagen XIα1.

Abstract

The extracellular matrix (ECM) plays an essential role in tumor progression and invasion through its continuous remodeling. The growth of most carcinomas is associated with an excessive collagen deposition that provides the proper environment for tumor development and chemoresistance. The α1 chain of a minor human collagen, type XI, is overexpressed in some tumor stroma, but not found in normal stroma. To test the clinical utility of this collagen as a cancer biomarker, specific receptors are needed. Available antibodies do not show enough selectivity or are directed toward the propeptide region that is cleaved when the protein is released to the ECM. Here we show the selection of an aptamer for the specific C-telopeptide region using a 16-mer peptide as the target for the SELEX. The aptamer selected with a Kd of ∼25 nM was able to capture the collagen XI from cell lysates. It was also used for target detection in a mixed antibody-aptamer sandwich assay showing it can be useful for diagnostic purposes in biological fluids.

• Authors: Ramón Lorenzo-Gómez, Rebeca Miranda-Castro, Juan R de Los Toyos, Noemí de-Los-Santos-Álvarez, María Jesús Lobo-Castañón
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 1189 Pg. 339206 (Jan 02 2022) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 34815029 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Aptamers, Nucleotide
• Biomarkers, Tumor
• COL11A1 protein, human
• Collagen Type XI

Topics

• Aptamers, Nucleotide
• Biomarkers, Tumor
• Collagen Type XI (analysis)
• Extracellular Matrix
• Humans
• Neoplasms (diagnosis)