Abstract | OBJECTIVE: To evaluate the antioxidant, anti- tumor and immunomodulatory activities of exopolysaccharides with different molecular masses isolated from Rhizopus nigricans. METHODS: Three polysaccharides with different molecular masses, namely RPS-1, RPS-2 and RPS-3, were separated from the fermentation broth of Rhizopus nigricans by fractional ethanol precipitation, and their capacity for scavenging DPPH, ABTS, and hydroxyl radicals was assessed. Cell counting kit-8 was used to analyze the changes in the viability of MFC, A549 and RAW 264.7 cells following treatments with the 3 polysaccharides; The level of nitric oxide in the supernatant of RAW 264.7 cells was detected using a nitric oxide detection kit, and the apoptosis rate of A549 cells was analyzed with flow cytometry. RESULTS: All the 3 polysaccharides had good antioxidant activities, and among them RPS-1 with a medium molecular mass exhibited the strongest scavenging capacity for DPPH and ABTS radicals (P < 0.05) while RPS-3 with the lowest molecular mass had the best scavenging activity for hydroxyl radicals (P < 0.01). All the 3 polysaccharides were capable of inhibiting the proliferation of MFC cells and A549 cells, activating the macrophages RAW 264.7 cells, and inducing apoptosis of A549 cells. RPS-2 with the highest molecular mass showed the strongest inhibitory effects against MFC and A549 cells (P > 0.05), and RPS-2 had the strongest activity for inducing apoptosis in A549 cells (P < 0.05). Compared with the other two polysaccharides, RPS-2 more strongly promoted the proliferation of RAW 264.7 cells and enhanced NO release from the cells (P < 0.05). CONCLUSION:
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Authors | Z Li, X Xu, Y Meng, Q Ma, F Huma, P Zhang, K Chen |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 41
Issue 10
Pg. 1540-1546
(Oct 20 2021)
ISSN: 1673-4254 [Print] China |
PMID | 34755670
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Polysaccharides
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Macrophages
- Mice
- Polysaccharides
(pharmacology)
- RAW 264.7 Cells
- Rhizopus
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