LncDACH1 promotes mitochondrial oxidative stress of cardiomyocytes by interacting with sirtuin3 and aggravates diabetic cardiomyopathy.

Abstract

Diabetic cardiomyopathy (DCM) is a common complication in diabetic patients. The molecular mechanisms of DCM remain to be fully elucidated. The intronic long noncoding RNA of DACH1 (lncDACH1) has been demonstrated to be closely associated with heart failure and cardiac regeneration. In this study, we investigated the role of lncDACH1 in DCM and the underlying molecular mechanisms. The expression of lncDACH1 was increased in DCM hearts and in high glucose-treated cardiomyocytes. Knockout of lncDACH1 reduced mitochondrial oxidative stress, cell apoptosis, cardiac fibrosis and hypertrophy, and improved cardiac function in DCM mice. Overexpression of lncDACH1 exacerbated mitochondria-derived reactive oxygen species (ROS) level and apoptosis, decreased activity of manganese superoxide dismutase (Mn-SOD); while silencing of lncDACH1 attenuated ROS production, mitochondrial dysfunction, cell apoptosis, and increased the activity of Mn-SOD in cardiomyocytes treated with high glucose. LncDACH1 directly bound to sirtuin3 (SIRT3) and facilitated its degradation by ubiquitination, therefore promoting mitochondrial oxidative injury and cell apoptosis in mouse hearts. In addition, SIRT3 silencing abrogated the protective effects of lncDACH1 deficiency in cardiomyocytes. In summary, lncDACH1 aggravates DCM by promoting mitochondrial oxidative stress and cell apoptosis via increasing ubiquitination-mediated SIRT3 degradation in mouse hearts. Inhibition of lncDACH1 represents a novel therapeutic strategy for the intervention of diabetic cardiomyopathy.

• Authors: Qi Zhang, Danyang Li, Xue Dong, Xiaowen Zhang, Junwu Liu, Lili Peng, Bo Meng, Qi Hua, Xinyu Pei, Lu Zhao, Xiaoxi Hu, Yang Zhang, Zhenwei Pan, Yanjie Lu, Baofeng Yang
• Journal: Science China. Life sciences (Sci China Life Sci) Vol. 65 Issue 6 Pg. 1198-1212 (06 2022) ISSN: 1869-1889 [Electronic] China
• PMID: 34668131 (Publication Type: Journal Article)
• Copyright: © 2021. Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.

Chemical References

• RNA, Long Noncoding
• Reactive Oxygen Species
• Sirt3 protein, mouse
• Superoxide Dismutase
• Sirtuin 3
• Glucose

Topics

• Animals
• Apoptosis (genetics)
• Diabetes Mellitus (metabolism)
• Diabetic Cardiomyopathies (genetics, metabolism)
• Glucose (pharmacology)
• Humans
• Mice
• Mice, Knockout
• Myocytes, Cardiac (metabolism)
• Oxidative Stress
• RNA, Long Noncoding (genetics)
• Reactive Oxygen Species (metabolism)
• Sirtuin 3 (genetics, metabolism)
• Superoxide Dismutase (genetics, metabolism)