Chronic rhinosinusitis with nasal polyposis (CRSwNP): the correlation between expression of Galectin-10 and Clinical-Cytological Grading (CCG).

Abstract

BACKGROUND:

Chronic rhinosinusitis with nasal polyps (CRSwNP) is typically characterized by Type 2 inflammation. Several biomarkers of eosinophilic inflammation, including Galectin-10, also known as Charcot-Leyden crystal protein (CLCP), have been identified to establish eosinophilic infiltration of polyps, a reliable predictor of recurrence.Objective: We aimed to evaluate the Galectin-10 expression in nasal polyps of patients with CRSwNP and to assess the correlation of Charcot-Leyden crystals expression to the severity of CRSwNP according to Clinical-Cytological Grading (CCG).

METHODS:

A double-label immunofluorescence was performed to evaluate the expression of Gal-10, CD15, Tryptase, and CD63 and their eventual co-localization on histological samples of 18 patients with CRSwNP. Double-positive Gal-10+CD15+ and Galectin-10+Tryptase+ inflammatory cells were counted by confocal microscopy.

RESULTS:

Galectin-10 was detectable in all examined tissues from CRSwNP patients, and its expression increased as low, medium and high CCG tissues were examined, respectively. Galectin-10 was extensively present in inflammatory cells, while limited Galectin-10 deposits were detected around mucosal epithelial cells.

CONCLUSION:

We showed the strong correlation between CCG and Galectin-10 expression, mainly colocalized with infiltrating eosinophils and mast-cells, in patients affected by CRSwNP.

• Authors: Matteo Gelardi, Giuseppe Stefano Netti, Rossana Giancaspro, Federica Spadaccino, Antonio Pennella, Valeria Fiore, Emanuel La Gatta, Gianluigi Mariano Grilli, Michele Cassano, Elena Ranieri
• Journal: American journal of rhinology & allergy (Am J Rhinol Allergy) Vol. 36 Issue 2 Pg. 229-237 (Mar 2022) ISSN: 1945-8932 [Electronic] United States
• PMID: 34647485 (Publication Type: Journal Article)

Chemical References

• Galectins
• Glycoproteins
• galectin 10, human
• Lysophospholipase
• lysolecithin acylhydrolase

Topics

• Chronic Disease
• Eosinophils
• Galectins (genetics)
• Glycoproteins
• Humans
• Lysophospholipase
• Nasal Polyps
• Rhinitis