Clinicopathological significance and underlying molecular mechanism of downregulation of basonuclin 1 expression in ovarian carcinoma.

Abstract

In this study, we aim to identify the clinical significance of basonuclin 1 (BNC1) expression in ovarian carcinoma (OV) and to explore its latent mechanisms. Via integrating in-house tissue microarrays, gene chips, and RNA-sequencing data, we explored the expression and clinical value of BNC1 in OV. Immunohistochemical staining was utilized to confirm the protein expression status of BNC1. A combined SMD of -2.339 (95% CI: -3.649 to -1.028, P < 0.001) identified that BNC1 was downregulated based on 1346 samples, and the sROC (AUC = 0.93) showed a favorable discriminatory ability of BNC1 in OV patients. We used univariate and multivariate Cox regulation to evaluate the prognostic role of BNC1 for OV patients, and a combined hazard ratio of 0.717 (95% CI: 0.445-0.989, P < 0.001) revealed that BNC1 was a protective factor for OV. Furthermore, the fraction of infiltrating naive B cells, memory B cells, and other immune cells showed statistical differences between the high- and low-BNC1 expression groups through cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm. Enrichment analysis showed that BNC1 may have a relationship with immune-related items in OV. By predicting the potential regulatory transcription factors (TFs) of BNC1, friend leukemia virus integration 1 (FLI1) may be a potential upstream TF of BNC1. Corporately, a decreasing trend of BNC1 may serve as a tumor suppressor and prognostic biomarker in OV patients. Moreover, BNC1 may take part in immune-related pathways and influence the fraction of tumor-infiltrating immune cells.

• Authors: Zi-Qian Liang, Lu-Yang Zhong, Jie Li, Jin-Hai Shen, Xin-Yue Tu, Zheng-Hong Zhong, Jing-Jing Zeng, Jun-Hong Chen, Zhu-Xin Wei, Yi-Wu Dang, Su-Ning Huang, Gang Chen
• Journal: Experimental biology and medicine (Maywood, N.J.) (Exp Biol Med (Maywood)) Vol. 247 Issue 2 Pg. 106-119 (01 2022) ISSN: 1535-3699 [Electronic] England
• PMID: 34644201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Transcription Factors
• Tumor Suppressor Proteins
• BNC1 protein, human

Topics

• DNA-Binding Proteins (immunology)
• Down-Regulation (immunology)
• Female
• Gene Expression Regulation, Neoplastic (immunology)
• Humans
• Lymphocytes, Tumor-Infiltrating (immunology, pathology)
• Memory B Cells (immunology, pathology)
• Ovarian Neoplasms (immunology, pathology)
• Transcription Factors (immunology)
• Tumor Suppressor Proteins (immunology)