Linkage of blood pressure, obesity and diabetes mellitus with angiotensinogen gene (AGT 704T>C/rs699) polymorphism in hypertensive patients.

Abstract

AIMS:

The study aim was to analyse the frequency of polymorphic variants of angiotensinogen gene polymorphism (AGT 704T>C, rs699) in essential arterial hypertension (EAH) patients.

METHODS:

Seventy-two individuals with EAH and hypertension‑mediated organ damage (stage 2), moderate, high or very high cardiovascular risks were involved in the case-control study. Among them, 70.84 % (51) were females and 29.16 % (21) were males; mean age was 59.87±7.98 y. The control group consisted of fifty practically healthy individuals at relevant age (49.13±6.28 y) and with relevant sex distribution (62 % were females, 38 % were males). AGT (704T>C) gene polymorphism was examined by RT-PCR.

RESULTS:

The distribution of genotypes in the study group was as follows: TT - 14 %, TC - 60 %, CC - 26 %, which corresponded to the distribution in the control group - 16 %, 54 % and 30 %, respectively, and did not deviate from the Hardy-Weinberg equilibrium. Smoking, type 2 diabetes mellitus (DM2) and obesity increased the relative risk of EAH in the examined population 2.5 times [OR=2.81; p=0.049], 3.75 times [OR=4.68; p=0.005] and almost twofold [OR=2.90; p=0.004], respectively. The probability of EAH increases fourfold with the angiotensin II elevation in the serum. Genotypes and alleles of the AGT (704T>C) gene were not significant risk factors for EAH and DM2 in the studied population. However, the TC-genotype (lesser T-allele) increases the risk of obesity in EAH patients more than 1.5 times [OR=2.93; p=0.03]. In addition, the T-allele increases the risk for blood pressure (BP) to elevate up to grade 2-3 [OR=3.64; p < 0.001].

CONCLUSIONS:

One-way ANOVA analysis confirmed the AGT (704T>C) gene polymorphism to be associated with systolic and diastolic BP elevation (F=7.80; p < 0.001 and F=4.90; p=0.01, respectively), especially in TT-genotype carriers (p < 0.05), and with body mass index increase, albeit only in women (F=13.94; p < 0.001) (Tab. 4, Fig. 3, Ref. 26). Text in PDF www.elis.sk Keywords: angiotensinogen gene (AGT 704T>C), diabetes mellitus type 2, arterial hypertension, obesity, risk.

• Authors: Y Repchuk, L P Sydorchuk, A R Sydorchuk, L Y Fedonyuk, O Kamyshnyi, O Korovenkova, I M Plehutsa, V S Dzhuryak, Y M Myshkovskii, O M Iftoda, R I Sydorchuk
• Journal: Bratislavske lekarske listy (Bratisl Lek Listy) Vol. 122 Issue 10 Pg. 715-720 ( 2021) ISSN: 0006-9248 [Print] Slovakia
• PMID: 34570572 (Publication Type: Journal Article)

Chemical References

• AGT protein, human
• Angiotensinogen

Topics

• Aged
• Angiotensinogen (genetics)
• Blood Pressure
• Case-Control Studies
• Diabetes Mellitus, Type 2
• Female
• Gene Frequency
• Genotype
• Humans
• Hypertension (epidemiology, genetics)
• Male
• Middle Aged
• Obesity (epidemiology, genetics)
• Polymorphism, Genetic