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DEAE-chitosan nanoparticles as a pneumococcus-biomimetic material for the development of antipneumococcal therapeutics.

Abstract
Advanced biomaterials provide an interesting and versatile platform to implement new and more effective strategies to fight bacterial infections. Chitosan is one of these biopolymers and possesses relevant features for biomedical applications. Here we synthesized nanoparticles of chitosan derivatized with diethylaminoethyl groups (ChiDENPs) to emulate the choline residues in the pneumococcal cell wall and act as ligands for choline-binding proteins (CBPs). Firstly, we assessed the ability of diethylaminoethyl (DEAE) to sequester the CBPs present in the bacterial surface, thus promoting chain formation. Secondly, the CBP-binding ability of ChiDENPs was purposed to encapsulate a bio-active molecule, the antimicrobial enzyme Cpl-711 (ChiDENPs-711), with improved stability over non-derivatized chitosan. The enzyme-loaded system released more than 90% of the active enzybiotic in ≈ 2 h, above the usual in vivo half-life of this kind of enzymes. Therefore, ChiDENPs provide a promising platform for the controlled release of CBP-enzybiotics in biological contexts.
AuthorsRoberto Vázquez, Francisco J Caro-León, Alberto Nakal, Susana Ruiz, Carmen Doñoro, Luis García-Fernández, Blanca Vázquez-Lasa, Julio San Román, Jesús Sanz, Pedro García, María Rosa Aguilar
JournalCarbohydrate polymers (Carbohydr Polym) Vol. 273 Pg. 118605 (Dec 01 2021) ISSN: 1879-1344 [Electronic] England
PMID34561005 (Publication Type: Journal Article)
CopyrightCopyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.
Chemical References
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Drug Carriers
  • diethylethylamine-chitosan
  • Chitosan
  • Endopeptidases
  • endolysin
Topics
  • A549 Cells
  • Anti-Bacterial Agents (chemistry, pharmacology)
  • Bacterial Proteins (metabolism)
  • Biomimetic Materials (chemistry, metabolism)
  • Chitosan (analogs & derivatives, chemistry, metabolism)
  • Drug Carriers (chemistry, metabolism)
  • Drug Liberation
  • Endopeptidases (chemistry, pharmacology)
  • Humans
  • Nanoparticles (chemistry, metabolism)
  • Streptococcus pneumoniae (drug effects)

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