Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified.

Abstract

Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer's lactate solution exhibited selective cancer-killing effects, and that the plasma-treated L-sodium lactate in the solution was an anti-tumor factor; however, the components that are generated through the interactions between plasma and L-sodium lactate and the components responsible for the selective killing of cancer cells remain unidentified. In this study, we quantified several major chemical products, such as pyruvate, formate, and acetate, in plasma-activated L-sodium lactate solution by nuclear magnetic resonance analysis. We further identified novel chemical products, such as glyoxylate and 2,3-dimethyltartrate, in the solution by direct infusion-electrospray ionization with tandem mass spectrometry analysis. We found that 2,3-dimethyltartrate exhibited cytotoxic effects in glioblastoma cells, but not in normal astrocytes. These findings shed light on the identities of the components that are responsible for the selective cytotoxic effect of plasma-activated solutions on cancer cells, and provide useful data for the potential development of cancer treatments using plasma-activated L-sodium lactate solution.

• Authors: Hiromasa Tanaka, Yugo Hosoi, Kenji Ishikawa, Jun Yoshitake, Takahiro Shibata, Koji Uchida, Hiroshi Hashizume, Masaaki Mizuno, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
• Journal: Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 18488 (09 16 2021) ISSN: 2045-2322 [Electronic] England
• PMID: 34531507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Author(s).

Chemical References

• Formates
• Glyoxylates
• Plasma Gases
• Tartrates
• formic acid
• Pyruvic Acid
• glyoxylic acid
• Sodium Lactate

Topics

• Brain Neoplasms (metabolism)
• Cell Death (drug effects)
• Cell Line, Tumor
• Formates (chemistry)
• Glioblastoma (metabolism)
• Glyoxylates (chemistry)
• Humans
• Plasma Gases (chemistry)
• Pyruvic Acid (chemistry)
• Sodium Lactate (chemistry)
• Tartrates (chemistry, toxicity)