To investigate the effects of
salt-inducible
kinase 2 (SIK2) on energy metabolism in rats with
cerebral ischemia-reperfusion. Adult SD male rats were divided into 5 groups:
sham group,
ischemia group, reperfusion group, adenovirus no-load group, and SIK2 overexpression group with 5 animals in each group. The
middle cerebral artery occlusion (MCAO) was induced with the modified Zea-Longa line
thrombus method to establish the
cerebral ischemia reperfusion model. Eight days before the MCAO, SIK2 overexpression was induced by injecting 7 μL adenovirus in the right ventricle, then MCAO was performed for followed by reperfusion HE staining was used to observe the pathological changes of cerebral tissue in rats; TTC staining was used to observe the volume of
cerebral infarct. The levels of
adenosine triphosphate (
ATP) and
adenosine diphosphate (
ADP) in rat brain tissue were detected by ELISA; the levels of SIK2 and
hypoxia-inducible factor 1α (HIF-1α) in the rat brain tissues were detected by RT-qPCR and Western blotting. Compared with the
sham group, SIK2 level was decreased in the
ischemia group, and it was further declined in the reperfusion group (<0.05). Compared with the
sham group and ischemic group, the pathological injury in reperfusion group were more severe, and the
infarct size was larger; compared with the reperfusion group and adenovirus no-load group, the pathological injury of the SIK2 overexpression group was milder, and the
infarct size is less. Compared with the sharn group, HIF-1α was increased in both
ischemia group and reperfusion group, especially in
ischemia group (all <0.05); HIF-1α level in the SIK2 overexpression group was higher than that in the reperfusion group and adenovirus no-load group (all <0.05).
ATP level in
ischemia group and reperfusion group was lower than that in the
sham group, and the reperfusion group decreased more significantly than the
ischemia group (<0.05);
ADP content was increased in the
ischemia and reperfusion group, and the
ADP content in reperfusion group was significantly higher than that in the
ischemia group (<0.05).
ATP level in the SIK2 overexpression group was higher than that in the reperfusion group and adenovirus no-load group (all <0.05), and
ADP was decreased in the SIK2 overexpression group (all <0.05). SIK2 can up-regulate the
ATP level and down-regulate the
ADP level in rat brain tissue and alleviate
cerebral ischemia-
reperfusion injury by increase the level of HIF-1α.