The immunomodulatory function of the porcine β-defensin 129: Alleviate inflammatory response induced by LPS in IPEC-J2 cells.

Abstract

β-defensin family plays a critical role in host defense against infections. In this study, we found that pBD129 are widely expressed in porcine tissues such as the intestine, liver, and spleen. Interestingly, the expression level of pBD129 in most tissues was higher in Tibetan pigs than in DLY (Duroc × Landrace × Yorkshire) pigs (P < 0.05), and was significantly upregulated upon E. coli K88 infection (P < 0.05). The pBD129 protein was successfully expressed in E. coli and the molecule weight was estimated by SDS-PAGE to be 37.2 kDa. Mass spectrometry verified the protein as a pBD129. The protein showed antibacterial activities against Streptococcus and E. coli DH5α with a minimal inhibitory concentration (MIC) of 32 μg/mL. Hemolytic and cytotoxicity assays indicated that pBD129 had no detrimental effect on cell viability. Importantly, pBD129 significantly reduced the apoptosis of porcine intestinal epithelial cells exposure to bacterial endotoxins, which was associated with down-regulation of inflammatory cytokines such as the IL-1β, IL-6 and TNFα (P < 0.05), and down-regulation of apoptosis-related genes such as the caspase-3, caspase-8, and caspase-9 (P < 0.05). These results suggested that pBD129 is a novel modulator of innate immunity involved in mammalian inflammatory responses.

• Authors: Kunhong Xie, Guoqi Su, Daiwen Chen, Bing Yu, Zhiqing Huang, Jie Yu, Ping Zheng, Yuheng Luo, Hui Yan, Hua Li, Jun He
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 188 Pg. 473-481 (Oct 01 2021) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 34352320 (Publication Type: Journal Article)
• Copyright: Copyright © 2021. Published by Elsevier B.V.

Chemical References

• Immunologic Factors
• Lipopolysaccharides
• beta-Defensins

Topics

• Animals
• Apoptosis (drug effects)
• Epithelial Cells (drug effects)
• Escherichia coli Infections (genetics, microbiology, therapy, veterinary)
• Gene Expression Regulation (drug effects)
• Immunologic Factors (genetics, pharmacology)
• Inflammation (chemically induced, genetics, pathology, therapy)
• Intestinal Mucosa (metabolism, microbiology)
• Lipopolysaccharides (toxicity)
• Microbial Sensitivity Tests
• Streptococcus (drug effects, pathogenicity)
• Swine
• beta-Defensins (genetics, pharmacology)