Assessing the potential value and mechanism of Ginkgo biloba L. On coal-fired arsenic-induced skin damage: In vitro and human evidence.

Abstract

Exposure through arsenic-contaminated air and food caused by the burning of coal is a major environmental public health concern in Guizhou Province of China. Previous studies have shown that immunological dysfunction is involved in the pathogenesis and carcinogenesis of arsenic; however, knowledge regarding effective prevention measures have not been fully examined. The effect of Ginkgo biloba extract (EGb761) on arsenic-induced skin damage of human immortalized keratinocyte cells (HaCaT) was first evaluated in this study. The results showed that 200 μg/mL EGb761 can reduce the expression of miR-155-5p, and the indicators reflecting arsenic-induced skin damage (Krt1, Krt6c and Krt10) in arsenic-exposed cells (P < 0.05), the expression levels of NF-AT1; the indicators reflecting arsenic-induced immunological dysfunction (IL-2, IFN-γ) in cells; and the levels of secreted IL-2 and IFN-γ in cell supernatants were significantly increased (P < 0.05). Further randomized controlled double-blind experiments showed that compared to the placebo control group, the expression level of miR-155-5p in the plasma of the Ginkgo biloba intervention group, the indicators in the serum reflecting arsenic-induced skin damage (Krt1, Krt6c, and Krt10) and the epithelial-mesenchymal transformation (EMT) vimentin were significantly reduced (P < 0.05), but the levels of NF-AT1 and the indicators reflecting arsenic-induced immunological dysfunction (IL-2, IFN-γ) and EMT (E-cadherin) in serum were significantly increased (P < 0.05). Our study provides some limited evidence that Ginkgo biloba L. can increase the expression of NF-AT1 by downregulating the level of miR-155-5p, alleviating immunological dysfunction, and decreasing the expression of EMT biomarkers, thus indirectly improving arsenic-induced skin damage.

• Authors: Qibing Zeng, Shaofeng Wei, Baofei Sun, Aihua Zhang
• Journal: Human & experimental toxicology (Hum Exp Toxicol) Vol. 40 Issue 12 Pg. 2113-2122 (Dec 2021) ISSN: 1477-0903 [Electronic] England
• PMID: 34085585 (Publication Type: Journal Article, Randomized Controlled Trial)

Chemical References

• IFNG protein, human
• IL2 protein, human
• Interleukin-2
• MIRN155 microRNA, human
• MicroRNAs
• NFATC Transcription Factors
• NFATC2 protein, human
• Plant Extracts
• Ginkgo biloba extract
• Interferon-gamma

Topics

• Adult
• Aged
• Arsenic Poisoning (blood, complications, drug therapy, genetics)
• Cell Line
• Cell Proliferation (drug effects)
• Double-Blind Method
• Female
• Ginkgo biloba
• Humans
• Interferon-gamma (blood, genetics)
• Interleukin-2 (blood, genetics)
• Keratinocytes (drug effects, metabolism)
• Male
• MicroRNAs (blood)
• Middle Aged
• NFATC Transcription Factors (blood, genetics, metabolism)
• Plant Extracts (pharmacology, therapeutic use)
• Skin Diseases (blood, chemically induced, drug therapy, genetics)