Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice.

Abstract

Obesity-induced adipose tissue dysfunction and disorders of glycolipid metabolism have become a worldwide research priority. Zfp217 plays a crucial role in adipogenesis of 3T3-L1 preadipocytes, but about its functions in animal models are not yet clear. To explore the role of Zfp217 in high-fat diet (HFD)-induced obese mice, global Zfp217 heterozygous knockout (Zfp217+/-) mice were constructed. Zfp217+/- mice and Zfp217+/+ mice fed a normal chow diet (NC) did not differ significantly in weight gain, percent body fat mass, glucose tolerance, or insulin sensitivity. When challenged with HFD, Zfp217+/- mice had less weight gain than Zfp217+/+ mice. Histological observations revealed that Zfp217+/- mice fed a high-fat diet had much smaller white adipocytes in inguinal white adipose tissue (iWAT). Zfp217+/- mice had improved metabolic profiles, including improved glucose tolerance, enhanced insulin sensitivity, and increased energy expenditure compared to the Zfp217+/+ mice under HFD. We found that adipogenesis-related genes were increased and metabolic thermogenesis-related genes were decreased in the iWAT of HFD-fed Zfp217+/+ mice compared to Zfp217+/- mice. In addition, adipogenesis was markedly reduced in mouse embryonic fibroblasts (MEFs) from Zfp217-deleted mice. Together, these data indicate that Zfp217 is a regulator of energy metabolism and it is likely to provide novel insight into treatment for obesity.

• Authors: Qianhui Zeng, Nannan Wang, Yaru Zhang, Yuxuan Yang, Shuangshuang Li, Rong Zheng, Jin Chai, Tong Qiao, Siwen Jiang
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 10 (May 20 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 34065474 (Publication Type: Journal Article)

Chemical References

• Trans-Activators
• ZFP217 protein, mouse

Topics

• Adipocytes, White (metabolism, physiology)
• Adipogenesis (physiology)
• Adipose Tissue, White (metabolism, physiopathology)
• Animals
• Diet, High-Fat
• Energy Metabolism (physiology)
• Fibroblasts (metabolism, physiology)
• Insulin Resistance (physiology)
• Lipid Metabolism (physiology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Mice, Obese
• Obesity (metabolism, physiopathology)
• Thermogenesis (physiology)
• Trans-Activators (metabolism)
• Weight Gain (physiology)