Nonsense-mediated mRNA decay efficiency influences bleeding severity in ITGA2B c.2659C > T (p.Q887X) knock-in mice.
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| Abstract |
Glanzmann's thrombasthenia (GT) is a severe hemorrhagic disease. It is caused by mutations in ITGA2B or ITGB3, which are the respective genes encoding integrin αIIb and β3. Despite widespread mutational analysis, the mechanisms underlying the extensive variability in bleeding severity observed among affected individuals remains poorly understood. In order to explore the mechanisms conferring for bleeding heterogeneity, three GT patients with ITGA2B c.2671C > T (p.Q891X) who possessed different bleeding scores were studied. Analysis showed that there was significant difference in nonsense-mediated mRNA decay (NMD) efficiency among the three patients. These differences positively correlated with their bleeding score. Next, a knock-in mouse model (KI mice) with the ITGA2B c.2659C > T (p.Q887X) was generated using CRISPR/Cas9. Importantly, this mutation is homologous to ITGA2B c.2671C > T (p.Q891X) in humans. The bleeding time of KI mice was significantly in comparison to the wide-type mice. Interestingly, bleeding was stopped after treatment with caffeine, which is a known NMD inhibitor. This suggests that NMD efficiency potentially influences bleeding severity in ITGA2B c.2659C > T (p.Q887X) KI mice.
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| Authors | Zhanli Xie, Jiang Jiang, Lijuan Cao, Miao Jiang, Fei Yang, Zhenni Ma, Zhaoyue Wang, Changgeng Ruan, Hong Liu, Lu Zhou |
| Journal | Clinical genetics (Clin Genet) Vol. 100 Issue 2 Pg. 213-218 (08 2021) ISSN: 1399-0004 [Electronic] Denmark |
| PMID | 33928629 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
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