Abstract |
Metronidazole is a drug used to treat bacterial and protozoan infections. Nowadays, it is one of the most frequently prescribed drugs worldwide. The main aim of this paper is to present a rapid, reliable and simple high-performance liquid chromatography (HPLC) method to determine metronidazole along with its primary metabolite, 2-hydroxymetronidazole, in plasma or serum using paracetamol as an internal standard. A total of 100% methanol was used to denature plasma proteins. After centrifugation, the supernatant was evaporated under nitrogen flow. The samples were dissolved in the mobile phase and injected into a Li-Chrospher RP-18 column. A total of 10 mmol/L NaH2PO4: acetonitrile (90:10, v/v) solution with a flow rate of 1 mL/min was used as the mobile phase. Metronidazole and 2-hydroxymetronidazole were detected at two different wavelengths at 320 nm and 311 nm, respectively. The method is characterized by high precision (relative standard deviation % < 6). The method was used for the determination of metronidazole and 2-hydroxymetronidazole in murine blood using small amounts of plasma (≤100 μL).
|
Authors | Nina Zemanová, Pavel Anzenbacher, Tomáš Hudcovic, Eva Anzenbacherová |
Journal | Journal of chromatographic science
(J Chromatogr Sci)
Vol. 60
Issue 1
Pg. 81-87
(Jan 01 2022)
ISSN: 1945-239X [Electronic] United States |
PMID | 33876238
(Publication Type: Journal Article)
|
Copyright | © The Author(s) 2021. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |
Chemical References |
- Metronidazole
- 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole
|
Topics |
- Animals
- Chromatography, High Pressure Liquid
- Metronidazole
(analogs & derivatives)
- Mice
- Plasma
|