In this study, we evaluated the effects of nanofiber and film
polymers with
doxycycline for treating a
wound in a diabetic rat model. 108 male rats were divided into six groups, the control group, the diabetic control, and the groups were diabetic rats receiving different
wound dressing. At the 3rd, 7th, and 14th days, macroscopic/histologic imaging and tissue sampling were performed. Tissues were analyzed for IL-1β, TNF-α,
IL-10,
TIMP-1, and MMP-2 by using ELISA. Dressings of
chitosan,
polyvinyl alcohol, and
doxycycline increased the rate of
wound closure, the volume of
collagen, dermal, and epidermis. In addition, it increased the number of fibroblasts and basal cell epidermis cells, vascular length, and decreased the number of neutrophil cells. Inflammatory
cytokines and MMP-2 were decreased, and anti-inflammatory
IL-10 and
TIMP-1 were increased. It was ultimately attained that the combination of
chitosan/
polyvinyl alcohol /
doxycycline could be a useful dressing for the healing of diabetic
wounds.