Abstract | BACKGROUND AND AIMS: METHODS AND RESULTS: When TGRL was labelled with fluorescent phospholipid 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), time- and dose-dependent transfer of DiI to HDL was observed upon incubations with lipoprotein lipase (LPL). The capacity of HDL to acquire DiI was decreased by -36% (p<0.001) in low HDL-C patients with acute myocardial infarction (n = 22) and by -95% (p<0.001) in low HDL-C subjects with Tangier disease (n = 7), unchanged in low HDL-C patients with Type 2 diabetes (n = 17) and in subjects with high HDL-C (n = 20), and elevated in subjects with extremely high HDL-C (+11%, p<0.05) relative to healthy normolipidemic controls. Across all the populations combined, HDL capacity to acquire DiI was directly correlated with HDL-C (r = 0.58, p<0.001). No relationship of HDL capacity to acquire DiI with both overall and cardiovascular mortality obtained from epidemiological studies for the mean HDL-C levels observed in the studied populations was obtained. CONCLUSIONS: These data indicate that the capacity of HDL to acquire phospholipid from TGRL upon LPL-mediated lipolysis is proportional to HDL-C and does not reflect cardiovascular risk in subjects widely differing in HDL-C levels.
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Authors | Feng Ma, Maryam Darabi, Marie Lhomme, Emilie Tubeuf, Aurélie Canicio, Jean Brerault, Narcisse Medadje, Fabiana Rached, Sandrine Lebreton, Eric Frisdal, Fernando Brites, Carlos Serrano, Raul Santos, Emmanuel Gautier, Thierry Huby, Petra El Khoury, Alain Carrié, Marianne Abifadel, Eric Bruckert, Maryse Guerin, Philippe Couvert, Philippe Giral, Philippe Lesnik, Wilfried Le Goff, Isabelle Guillas, Anatol Kontush |
Journal | Atherosclerosis
(Atherosclerosis)
Vol. 324
Pg. 1-8
(05 2021)
ISSN: 1879-1484 [Electronic] Ireland |
PMID | 33798922
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Lipoproteins, HDL
- Phospholipids
- Triglycerides
- Cholesterol
- Lipoprotein Lipase
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Topics |
- Cardiovascular Diseases
(diagnosis)
- Cholesterol
- Diabetes Mellitus, Type 2
- Heart Disease Risk Factors
- Humans
- Lipolysis
- Lipoprotein Lipase
(metabolism)
- Lipoproteins, HDL
(metabolism)
- Phospholipids
- Risk Factors
- Triglycerides
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