Lentinan can lead to apoptosis of
tumor cells and improve immune function. However, limited research focused on the immunogenic death regulation mechanism of
lentinan on mouse H22 cells. The study aimed to explore the effect of
Lentinan on the expression of immunogenic death-related
proteins in mice H22 cells. MTT method was used to detect and evaluate the effect of 200-1000 μg/mL
lentinan on the survival rate of H22 cells after 24 h, 48 h, and 72 h, respectively. Flow cytometry was employed to collect the apoptotic rate of
lentinan at different concentrations (200-800μg/mL) on H22 cells for 48 h, and obtain the apoptotic rate of 600 μg/mL
lentinan at different times (12-72 h). The effect of
Lentinan on the expression of H22 Immunogenic Cell Death
proteins was analyzed by ELISA and HPLC-MS afterward. Results suggest that
lentinan cytotoxic and pro-apoptotic have a concentration-dependent manner with the H22 cells. Moreover, the rate of apoptosis increased significantly (P < 0.05) in 24 h.
Lentinan can induce the expression of
Calreticulin(CRT), High mobility
protein 1(
HMGB1),
ATP and
Heat shock protein 70 (HSP70) .Therefore, the antitumor effect of
lentinan may be related to the regulation of immunogenic death-related
protein expression, which was beneficial to the future development of
liver cancer vaccines.