Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: METHODS: DSS-induced UC mice were established and randomly divided into the following four groups: control group, DSS group, Rh2 (50 mg/kg) group and sulfasalazine (SASP, 200 mg/kg) group. Except for the control group, 3% DSS drinking water was given to each group for 7 days, and the other two groups were intragastrically administered with Rh2 and SASP for 10 days. At the end of the experiment, colon samples were collected, and phenotypic and pathological analyses were performed in UC mice. Then, Western blot, immunohistochemistry and quantitative real-time PCR analyses were performed to determine the expression of signaling pathway-related factors. RESULTS: Rh2 markedly alleviated DSS-induced body weight loss, intestinal damage, colon length shortening and disease activity index (DAI) scores. Furthermore, proinflammatory cytokines, such as TNF-α, IL-6 and IL-1β, were reduced by Rh2. Additionally, STAT3/miR-214 activation was also suppressed by Rh2 administration. In vitro, we demonstrated that Rh2 effectively inhibited IL-6-induced STAT3 phosphorylation and miR-214 expression in cultured normal colonic epithelial cells. CONCLUSION: Our results suggested that Rh2 exhibits potential application value in the treatment of UC, and its mechanism is related to the downregulation of STAT3/miR-214 levels, which is expected to be applicable in the treatment of clinical UC.
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Authors | Xuanqing Chen, Tingting Xu, Xiangyu Lv, Jingwei Zhang, Shijia Liu |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 274
Pg. 113997
(Jun 28 2021)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 33705918
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Cytokines
- Ginsenosides
- MIRN214 microRNA, human
- MicroRNAs
- Mirn214 microRNA, mouse
- STAT3 Transcription Factor
- STAT3 protein, human
- Stat3 protein, mouse
- Sulfasalazine
- ginsenoside Rh2
- Dextran Sulfate
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Cell Line
- Colitis, Ulcerative
(chemically induced, drug therapy, metabolism, pathology)
- Cytokines
(metabolism)
- Dextran Sulfate
(toxicity)
- Disease Models, Animal
- Ginsenosides
(chemistry, pharmacology, therapeutic use)
- Humans
- Inflammation
(chemically induced, drug therapy, metabolism)
- Male
- Mice, Inbred C57BL
- MicroRNAs
(antagonists & inhibitors, metabolism)
- STAT3 Transcription Factor
(antagonists & inhibitors, metabolism)
- Signal Transduction
(drug effects)
- Sulfasalazine
(pharmacology, therapeutic use)
- Mice
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