Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro.

Abstract

OBJECTIVE:

To investigate the regulation of psoriatic dermal mesenchymal stem cells (p-DMSCs) in the expression of vascular growth factor (VEGF), and migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro.

METHODS:

A co-culture model of HUVECs and dermal mesenchymal stem cells (DMSCs)was used in this study. After 7-day co-culture, changes in expression levels of VEGF mRNA and protein in HUVECs were assessed using RT-PCR and Western Blotting, respectively. Migration and tubular formation of HUVECs were also assessed following co-culture of DMSCs and HUVECs.

RESULTS:

In comparison to either HUVECs alone or co-culture of n-DMSCs and HUVECs, co-culture of HUVECs and p-DMSCs significantly increased expression levels of both VEGF mRNA (p < 0.01 vs. HUVECs alone) and protein in HUVECs (p < 0.001 vs. both HUVECs alone and HUVECs co-cultured with n-DMSCs). Moreover, p-DMSCs stimulated HUVEC migration and vascular formation (p < 0.05 vs. both HUVECs alone and co-culture of n-DMSCs and HUVECs).

CONCLUSION:

Psoriatic DMSCs can upregulate VEGF expression, and stimulate migration and angiogenesis of HUVECs, suggesting a pathogenic role of p-DMSCs in psoriasis.

• Authors: Ling Zhou, Juanjuan Wang, Jiannan Liang, Hui Hou, Jiao Li, Juan Li, Yue Cao, Junqin Li, Kaiming Zhang
• Journal: Microvascular research (Microvasc Res) Vol. 136 Pg. 104151 (07 2021) ISSN: 1095-9319 [Electronic] United States
• PMID: 33662409 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021. Published by Elsevier Inc.

Chemical References

• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Cell Communication
• Cell Movement
• Cell Proliferation
• Cells, Cultured
• Coculture Techniques
• Human Umbilical Vein Endothelial Cells (metabolism)
• Humans
• Mesenchymal Stem Cells (metabolism, pathology)
• Neovascularization, Physiologic
• Psoriasis (metabolism, pathology)
• Signal Transduction
• Skin (metabolism, pathology)
• Up-Regulation
• Vascular Endothelial Growth Factor A (genetics, metabolism)