Development of a novel fluorescent biosensor for dynamic monitoring of metabolic methionine redox status in cells and tissues.
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| Abstract |
Aberrant production of reactive oxygen species (ROS) leads to tissue damage accumulation, which is associated with a myriad of human pathologies. Although several sensors have been developed for ROS quantification, their applications for ROS-related human physiologies and pathologies still remain problematic due to the unstable nature of ROS. Herein, we developed Trx1-cpYFP-fRMsr (TYfR), a genetically-encoded fluorescent biosensor with the remarkable specificity and sensitivity toward fMetRO (free Methionine-R-sulfoxide), allowing for dynamic quantification of physiological levels of fMetRO, a novel indicator of ROS and methionine redox status in vitro and in vivo. Moreover, using the sensor, we observed a significant fMetRO enrichment in serum from patients with acute coronary syndrome, one of the most severe cardiovascular diseases, which becomes more evident following percutaneous coronary intervention. Collectively, this study proposes that fMetRO is a novel biomarker of tissue damage accumulation in ROS-associated human pathologies, and that TYfR is a promising tool for quantifying fMetRO with potentials in versatile applications.
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| Authors | Dong Wook Choi, Yeon Jin Roh, Seahyun Kim, Hae Min Lee, Minseo Kim, Donghyuk Shin, Jong Ho Park, Yongmin Cho, Hee Ho Park, Yong Sik Ok, Donghyun Kang, Jin-Hong Kim, Lionel Tarrago, Nika N Danial, Vadim N Gladyshev, Pil-Ki Min, Byung Cheon Lee |
| Journal | Biosensors & bioelectronics (Biosens Bioelectron) Vol. 178 Pg. 113031 (Apr 15 2021) ISSN: 1873-4235 [Electronic] England |
| PMID | 33571808 (Publication Type: Journal Article) |
| Copyright | Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved. |
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