An accurate, high-speed, portable bifunctional electrical detector for COVID-19.

Abstract

Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread and caused a severe global pandemic. Because no specific drugs are available for COVID-19 and few vaccines are available for SARS-CoV-2, accurate and rapid diagnosis of COVID-19 has been the most crucial measure to control this pandemic. Here, we developed a portable bifunctional electrical detector based on graphene fieldeffect transistors for SARS-CoV-2 through either nucleic acid hybridization or antigen-antibody protein interaction, with ultra-low limits of detection of ~0.1 and ~1 fg mL-1 in phosphate buffer saline, respectively. We validated our method by assessment of RNA extracts from the oropharyngeal swabs of ten COVID-19 patients and eight healthy subjects, and the IgM/IgG antibodies from serum specimens of six COVID-19 patients and three healthy subjects. Here we show that the diagnostic results are in excellent agreement with the findings of polymerase chain reaction-based optical methods; they also exhibit rapid detection speed (~10 min for nucleic acid detection and ~5 min for immunoassay). Therefore, our assay provides an efficient, accurate tool for high-throughput point-of-care testing.

ELECTRONIC SUPPLEMENTARY MATERIAL:

Supplementary material is available in the online version of this article at 10.1007/s40843-020-1577-y.

• Authors: Guojun Ke, Dingkai Su, Yu Li, Yu Zhao, Honggang Wang, Wanjian Liu, Man Li, Zhiting Yang, Fang Xiao, Yao Yuan, Fei Huang, Fanyang Mo, Peng Wang, Xuefeng Guo
• Journal: Science China materials (Sci China Mater) Vol. 64 Issue 3 Pg. 739-747 ( 2021) ISSN: 2095-8226 [Print] China
• PMID: 33552629 (Publication Type: Journal Article)
• Copyright: © Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020.