Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds.

Abstract

This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.

• Authors: Yosuke Niimi, Dannelys Pérez-Bello, Koji Ihara, Satoshi Fukuda, Sam Jacob, Clark R Andersen, Tuvshintugs Baljinnyam, Jisoo Kim, Suzan Alharbi, Donald S Prough, Perenlei Enkhbaatar
• Journal: Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 975 (01 13 2021) ISSN: 2045-2322 [Electronic] England
• PMID: 33441597 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fish Oils
• 3-nitrotyrosine
• Tyrosine
• Telomerase

Topics

• 3T3 Cells
• Animals
• Burns (drug therapy, metabolism)
• Cell Line
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Disease Models, Animal
• Female
• Fish Oils (pharmacology)
• Hippophae (metabolism)
• Keratinocytes (drug effects, metabolism)
• Mice
• Re-Epithelialization (drug effects)
• Sheep
• Telomerase (metabolism)
• Tyrosine (analogs & derivatives, metabolism)
• Wound Healing (drug effects)