Abstract |
Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 different serotypes have been reported to be circulating across the various agro-climatic zones of the country. Non-structural proteins (NSPs) of bluetongue virus have always remained ideal target for differentiation of infected from vaccinated animals. The current study is an extrapolation of our previous work where a novel fusion construct comprising of bluetongue viral segment NS1 and NS3 was successfully cloned, expressed, purified with an efficient strategy for its suitable implementation as a diagnostic antigen. In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue.
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Authors | Nihar Nalini Mohanty, Divakar Hemadri, Archana Munivenkatarayappa, Namrata Shetty, Vinutha Subramanyam, Sanchay Kumar Biswas, Mohammed Mudassar Chanda, Sathish B Shivachandra |
Journal | Journal of immunological methods
(J Immunol Methods)
Vol. 490
Pg. 112959
(03 2021)
ISSN: 1872-7905 [Electronic] Netherlands |
PMID | 33412173
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Antibodies, Viral
- NS1 protein, bluetongue virus
- Recombinant Fusion Proteins
- S10 protein, Bluetongue virus
- Viral Nonstructural Proteins
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Topics |
- Animals
- Antibodies, Viral
(blood)
- Bluetongue
(diagnosis, immunology)
- Bluetongue virus
(physiology)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Immunity, Humoral
- Recombinant Fusion Proteins
(genetics, metabolism)
- Sheep
(immunology, virology)
- Viral Nonstructural Proteins
(genetics, metabolism)
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