Abstract | AIM: To date, various molecules have been investigated to reduce the effect of renal ischemia/reperfusion (I/R) injury. However, none have yet led to clinical use. The present study aimed to investigate the protective effect of cordycepin (C) on renal I/R injury in an experimental rat model. MATERIALS AND METHODS: Twenty-four mature Sprague Dawley female rat was randomly divided into three groups: Sham, I/R, I/R+C. All animals underwent abdominal exploration. To induce I/R injury, an atraumatic vascular bulldog clamp was applied to the right renal pedicle for 60 minutes ( ischemia) and later clamp was removed to allow reperfusion in all rats, except for the sham group. In the I/R + C group, 10 mg/kg C was administered intraperitoneally, immediately after reperfusion. After 4 hours of reperfusion, the experiment was terminated with right nephrectomy. Histological studies and biochemical analyses were performed on the right nephrectomy specimens. EGTI (endothelial, glomerular, tubulointerstitial) histopathology scoring and semi-quantitative analysis of renal cortical necrosis were used for histological analyses and superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total oxidant status (TOS) for biochemical analyses. RESULTS: Histopathological examination of the tissue damage revealed that all kidneys in the sham group were normal. The I/R group had higher histopathological scores than the I/R + C group. In the biochemical analysis of the tissues, SOD, MDA, TOS values were found to be statistically different in the I/R group compared to the I/R + C group (p: 0.004, 0.004, 0.001 respectively). CONCLUSIONS: Intraperitoneal cordycepin injection following ischemia preserve renal tissue against oxidative stress in a rat model of renal I/R injury.
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Authors | Hasan Riza Aydin, Cagri Akin Sekerci, Ertugrul Yigit, Hatice Kucuk, Huseyin Kocakgol, Seyfi Kartal, Yiloren Tanidir, Orhan Deger |
Journal | Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica
(Arch Ital Urol Androl)
Vol. 92
Issue 4
(Dec 18 2020)
ISSN: 2282-4197 [Electronic] Italy |
PMID | 33348963
(Publication Type: Journal Article)
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Chemical References |
- Deoxyadenosines
- cordycepin
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Topics |
- Animals
- Deoxyadenosines
(therapeutic use)
- Female
- Kidney
(blood supply)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
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