Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury.

Abstract

This study explores the effects of oxytocin receptor (OXTR) in the trigeminal ganglion (TG) on orofacial neuropathic pain. We demonstrate that OXTR activation in the TG relieves the orofacial ectopic pain as well as inhibits the upregulated expression of calcitonin gene-related peptide (CGRP), IL-1β, and TNFα in the TG and spinal trigeminal nucleus caudalis (SpVc) of rats with inferior alveolar nerve transection. OXTR, a G protein-coupled receptor, has been demonstrated to play a significant role in analgesia after activation by its canonical agonist oxytocin (OXT) in the dorsal root ganglion. However, the role of OXTR in the trigeminal nervous system on the orofacial neuropathic pain is still little known. In the present study, we aimed to investigate the regulation effect and mechanism of OXTR in the TG) and SpVc) on orofacial ectopic pain induced by trigeminal nerve injury. The inferior alveolar nerve (IAN) was transected to establish a ectopic pain model. A behavioral test with electronic von Frey filament demonstrated IAN transection (IANX) evoked mechanical hypersensitivity in the whisker pad from day 1 to at least day 14 after surgery. In addition, administration of OXT (50 and 100 μM) into the TG attenuated the mechanical hypersensitivity induced by IANX, which was reversed by pretreatment with L-368,899 (a selective antagonist of OXTR) into the TG. In addition, immunofluorescence showed the expression of OXTR in neurons in the TG and SpVc. Furthermore, Western blot analysis indicated that the upregulated expression of OXTR, CGRP, IL-1β, and TNFα in the TG and SpVc after IANX was inhibited by the administration of OXT into the TG. And the inhibition effect of OXT on the expression of CGRP, IL-1β, and TNFα was abolished by preapplication of OXTR antagonist L-368,899 into the TG.NEW & NOTEWORTHY This study explores the effects of oxytocin receptor (OXTR) in the trigeminal ganglion (TG) on orofacial neuropathic pain. We demonstrate that OXTR activation in the TG relieves the orofacial ectopic pain as well as inhibits the upregulated expression of calcitonin gene-related peptide, IL-1β, and TNF-α in the TG and spinal trigeminal nucleus caudalis of rats with inferior alveolar nerve transection.

• Authors: Chao-Lan Huang, Fei Liu, Yan-Yan Zhang, Jiu Lin, Min Fu, Yue-Ling Li, Cheng Zhou, Chun-Jie Li, Jie-Fei Shen
• Journal: Journal of neurophysiology (J Neurophysiol) Vol. 125 Issue 1 Pg. 223-231 (01 01 2021) ISSN: 1522-1598 [Electronic] United States
• PMID: 33326336 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Camphanes
• Interleukin-1beta
• Piperazines
• Receptors, Oxytocin
• Tumor Necrosis Factor-alpha
• Oxytocin
• L 368899
• Calcitonin Gene-Related Peptide

Topics

• Animals
• Calcitonin Gene-Related Peptide (metabolism)
• Camphanes (pharmacology)
• Interleukin-1beta (metabolism)
• Male
• Mandibular Nerve Injuries (metabolism, physiopathology)
• Oxytocin (metabolism, therapeutic use)
• Pain (drug therapy, etiology)
• Piperazines (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Oxytocin (agonists, antagonists & inhibitors, metabolism)
• Trigeminal Ganglion (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)