A simple HPLC assay for determining eltrombopag concentration in human serum.

Abstract

Eltrombopag, a thrombopoietin receptor agonist, is used for the treatment of idiopathic thrombocytopenic purpura (ITP) and aplastic anemia. We developed a HPLC assay for the determination of serum eltrombopag concentration in ITP patients. An aliquot of a serum sample spiked with diclofenac as the internal standard (IS) was treated with acetonitrile to precipitate the proteins. Eltrombopag and the IS were separated on an octadecylsilyl silica-gel column using a mobile phase consisting of 10 mM 1-pentanesulfonic acid sodium salt, acetonitrile, and acetic acid. The detection wavelength was set at 265 nm. The calibration curve was linear at the concentration range of 0.15-12.5 μg/mL for eltrombopag (r = 0.9987). The recoveries of eltrombopag from the serum samples were greater than 95.9% with coefficients of variation (CVs) being less than 2.8%. The CVs for the intra-day and inter-day assays were 1.9-11.8% and 1.0-11.8%, respectively. This assay method could be used for therapeutic drug monitoring of eltrombopag in ITP patients.

• Authors: Naoya Yanagimachi, Naoshi Obara, Mamiko Sakata-Yanagimoto, Shigeru Chiba, Kosuke Doki, Masato Homma
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 35 Issue 5 Pg. e5049 (May 2021) ISSN: 1099-0801 [Electronic] England
• PMID: 33314287 (Publication Type: Journal Article)
• Copyright: © 2020 John Wiley & Sons, Ltd.

Chemical References

• Benzoates
• Hydrazines
• Pyrazoles
• Receptors, Thrombopoietin
• MPL protein, human
• Diclofenac
• eltrombopag

Topics

• Benzoates (administration & dosage, blood)
• Chromatography, High Pressure Liquid (methods)
• Diclofenac (standards)
• Drug Monitoring
• Humans
• Hydrazines (administration & dosage, blood)
• Purpura, Thrombocytopenic, Idiopathic (drug therapy)
• Pyrazoles (administration & dosage, blood)
• Receptors, Thrombopoietin (antagonists & inhibitors)
• Reference Standards